Index-> contents reference index search external Previous Next Home home-> links software journals packages publications resume pub_abstract white_paper ssh_key fedora_package bikelist rock_climb picture mdot_bike_wish pub_abstract-> newton_step tmb rkhs_bayes dismod_pde sskm l1_rks implicit_ad ckbs bayes_inverse mcmc_smooth semiblind neuron ConsumeO2 StochasticNonlinear SmoothVariance randompar markov sammii relative gps smoother prolate update nltrack network burg semi gamma match nsmooth error line_search ConsumeO2 Headings-> Abstract Keywords

Estimating In Vitro Mitochondrial Oxygen Consumption During Muscle Contraction and Recovery: A Novel Approach that Accounts for Diffusion

Abstract
A deconvolution algorithm, based on a Bayesian statistical framework and smoothing spline technique, is applied to reconstructing input functions from noisy measurements in biological systems. Deconvolution is usually ill-posed. However, placing a Bayesian prior distribution on the input function can make the problem well-posed. Using this algorithm and a computational model of diffusional oxygen transport in an approximately cylindrical muscle (about 0.5 mm diameter and 10 mm long mouse leg muscle), the time course of muscle oxygen uptake and mitochondrial oxygen consumption, both during isometric twitch contractions (at various frequencies) and the recovery period, is estimated from polarographic measurements of oxygen concentration on the muscle surface. An important feature of our experimental protocol is the availability of data for the apparatus characteristics. From these time courses, the actual mitochondrial consumption rates during resting and exercise states can be estimated. Mitochondrial oxygen consumption rate increased during stimulation to a maximum steady state value approximately 5 times of the resting value of 0.63 nanomoles per second per gram-wet weight for the stimulation conditions studied. Diffusion slowed the kinetic responses to the contraction but not the steady state fluxes during the stimulation interval.

Keywords
Muscle contraction, Muscle oxygen uptake, Mitochondrial oxygen consumption, Muscle energetics, Oxygen diffusion, Deconvolution.  citation
Input File: ConsumeO2.omh